## Linear Regression: EPT Relative Abundance vs. Percent Sands and Fines for Minnesota Streams

### Plot

- Analysis: Linear regression
- Independent variable: Percentage (%), sand and fines
- Dependent variable: EPT (Ephemeroptera, Plecoptera and Trichoptera) abundance / m²
- Classification: Using Omernik level II Ecoregions with dummy variable = 0 for ecoregions 46, 47, and 51 and dummy variable = 1 for Ecoregions 50 and 52.
- Key: symbols = observations, solid line = mean line, dashed line = 95% prediction limit. Black lines and symbols: Ecoregions 46, 47 and 51, blue lines and symbols: Ecoregions 50 and 52
- Sample size: n = 267

### Model

- Ecoregions 50 and 52: log
_{e}(EPT Abundance + 1) = 5.096 - 0.0080(% sand & fines) - Ecoregions 46, 47 and 51: log
_{e}(EPT Abundance + 1) = 5.442 - 0.0170 (% sand & fines) - r² = 0.181
- Date: 6 October, 2006

Variable | Estimate | t value | p |
---|---|---|---|

Intercept | 5.442 | 31.27 | |

Ecoregion class | -0.346 | -1.54 | 0.12 |

% Sand & fines | -0.0170 | -6.40 | <0.0001 |

% Sand & fines x Ecoregion class | -0.00110 | 2.68 | 0.008 |

### Data Analysis

- EPT abundance was transformed by taking the natural log of abundance +1 for each observation.
- Model generated using SAS Proc GLM with the solution option. The 95% prediction limits were calculated using the equation in step 9 of box 14.3 from Sokal and Rohlf (1995). The intercept, slope, grand mean of % sand & fines, and corrected sums of squares were calculated using Proc REG and Proc UNIVARIATE.
- The ecoregions were aggregated into two classes because a preliminary ANOVA indicated that the base material for bedded sediments, % coarse substrates, was significantly greater in Ecoregions 50 and 52 than in Ecoregions 46, 47, and 51. Percent coarse substrates, however, did not significantly differ within either group.
- Replicate visits to a site were excluded from the data set.

### Data Origin

- Agency: Minnesota Pollution Control Agency , St. Paul, Minnesota
- Location: Minnesota - analyses restricted to wadeable sites (52 km² < watershed area < 523 km²) from the five ecoregions indicated below.
- Omernik Level III Ecoregions examined include: 46 (Northern Glaciated Plains), 47 (Western Corn Belt Plains), 50 (Northern Lakes and Forests), 51 (North Central Hardwoods), and 52 (Driftless Area).

### Sampling Design

- Data collected 1996 through 2004.
- Sampling protocols:
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### Contact

Michael B. Griffith, griffith.michael@epa.gov, (513) 569-7034